Genotyping by Sequencing for SNP-Based Linkage Map Construction and QTL Analysis of Chilling Requirement and Bloom Date in Peach [Prunus persica (L.) Batsch]
نویسندگان
چکیده
Low-cost, high throughput genotyping methods are crucial to marker discovery and marker-assisted breeding efforts, but have not been available for many 'specialty crops' such as fruit and nut trees. Here we apply the Genotyping-By-Sequencing (GBS) method developed for cereals to the discovery of single nucleotide polymorphisms (SNPs) in a peach F2 mapping population. Peach is a genetic and genomic model within the Rosaceae and will provide a template for the use of this method with other members of this family. Our F2 mapping population of 57 genotypes segregates for bloom time (BD) and chilling requirement (CR) and we have extensively phenotyped this population. The population derives from a selfed F1 progeny of a cross between 'Hakuho' (high CR) and 'UFGold' (low CR). We were able to successfully employ GBS and the TASSEL GBS pipeline without modification of the original methodology using the ApeKI restriction enzyme and multiplexing at an equivalent of 96 samples per Illumina HiSeq 2000 lane. We obtained hundreds of SNP markers which were then used to construct a genetic linkage map and identify quantitative trait loci (QTL) for BD and CR.
منابع مشابه
First genetic linkage map of chilling injury susceptibility in peach (Prunus persica (L.) Batsch) fruit with SSR and SNP markers
Peach and nectarine (Prunus persica L) are highly perishable; they ripen and deteriorate quickly at ambient temperature. Storage at low temperature (0–5oC) is a common strategy used to slow the ripening processes and extending shelf life. However, if susceptible varieties are held too long at a low temperature, they will not ripen properly and will develop chilling injury (CI) symptoms like mea...
متن کاملAssociation genetics of chilling injury susceptibility in peach (Prunus persica (L.) Batsch) across multiple years
Peach and nectarine (Prunus persica L.) are highly perishable; they ripen and deteriorate quickly at ambient temperature. Storage at low temperature (0-5 °C) is a common strategy used to slow the ripening processes and to extend shelf life. However, if susceptible varieties are held too long at a low temperature, they will not ripen properly and will develop chilling injury (CI) symptoms like m...
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Promising new tools for peach fruit quality breeding have been revised in this work. These tools included fruit quality evaluation through physical, chemical and sensorial parameters and post-harvest storage evaluation. The development of a feasible method for an early testing of peach selections generated in breeding programs is also well described together with the discussion of the inheritan...
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Mapping and sequencing of the non-dormant evg mutant in peach [Prunus persica (L.) Batsch] identified six tandem-arrayed DAM (dormancy-associated MADS-box) genes as candidates for regulating growth cessation and terminal bud formation. To narrow the list of candidate genes, an attempt was made to associate bud phenology with the seasonal and environmental patterns of expression of the candidate...
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عنوان ژورنال:
دوره 10 شماره
صفحات -
تاریخ انتشار 2015